Skin Irritation Test

What Does Irritation Mean?

Irritation can be defined as a condition of the mucous membranes and skin caused by irritants.

What is the Importance of Irritation Tests?

The chemical components used in the formulation of biocidal products may cause irritant effects when in direct contact with the skin, eyes or respiratory tract. Therefore, biocidal products should be subjected to irritation tests before being placed on the market. Irritation analysis is performed to identify potentially harmful effects of the product, assess risks and ensure user safety.

Scope of Irritation Tests

Irritation tests are usually performed in three main categories: skin irritation, eye irritation and respiratory irritation.

1. Skin Irritation Test:
   • Test Method: This test determines whether biocidal products cause any skin reactions such as irritation, redness or swelling as a result of skin contact.
   • Human or Animal Testing: With the development of alternative methods (in vitro testing, computer modeling), alternative approaches to animal testing are also being used. However, from a safety point of view, such tests are still valid.
   • Method: One of the most widely used tests is OECD Test Guideline 404, where the effects of the product on the skin are observed for 24-72 hours.
2. Eye Irritation Test:
   • Test Method: It is a test to determine the irritation and potential harmful effects of biocidal products in the eye as a result of eye contact. Symptoms such as redness, swelling, watering and pain in the eye are observed.
   • OECD Test Guideline 405: Eye irritation tests are usually performed in accordance with this guideline. This test assesses how severe the effects of the product are in the eye.
3. Respiratory Irritation Test:
   • Test Method: When biocidal products are used in aerosol form, it is a test to determine whether these products cause irritation to the respiratory tract. Respiratory tract irritation may be particularly important in contact with aerosols such as gases, vapors or sprays.
   • Testing Process: Respiratory irritation tests can be performed with animal models or in vitro tests.

What are the Methods Used in Irritation Analysis?

1. In Vivo Tests: These are tests carried out on humans or animals. In these tests, biocidal products come into direct contact with the skin, eyes or respiratory tract and the effects are observed.
2. In Vitro Tests: Laboratory cell culture or tissue models are used to evaluate the irritant effects of biocidal products. This is an alternative approach that helps to avoid the use of animals.

In our laboratories serving in many parts of Turkey, "Irritation Test" is performed in accordance with legal regulations and standards related to TÜRKAK accreditation.

OECD 439

Irritation Test / Skin Irritation and Corrosion Toxicity