ADF, NDF and ADL Analysis in Feed

Structural and organic acids as cellulose, lignin and hemicellulose, and forages consisting of non-structural carbohydrates such as sugar are used in animal nutrition. Monogastric animals cannot digest these structural carbohydrates in roughage. Carbohydrates in roughage are divided into two groups:

NDF (cellulose, hemicellulose and lignin)
ADF (cellulose, hemicellulose)

The importance of using structural carbohydrates in animal feed is to increase feed efficiency and protect rumen health in ruminants. With the intake of NDF and ADF, saliva secretion and rumen pH remain within appropriate limits in ruminants. Thus, it provides a suitable environment for cellulotic and amylolytic bacteria, protozoa and yeasts that help microbial digestion. In addition, many diseases such as acidosis, laminitis, rumen paraketosis are prevented with NDF and ADF.

Due to the reasons mentioned above, the determination of the amount of cellulose – crude cellulose in feeds is one of the important analyzes. For many years, the amount of fiber in feeds was determined by "Raw Cellulose Analysis". The most used methods today are:

Neutral Detergent Insoluble Fiber (NDF) - hemicellulose, cellulose and lignin
Acid Detergent Insoluble Fiber (ADF) - cellulose and lignin
Acid Detergent Lignin (ADL) - lignin

“ADF, NDF and ADL Determination” is carried out on feeds in accredited and authorized Food Analysis Laboratories.

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