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C4 Sugar Analysis is performed to determine whether honey is mixed with sugars. This internationally accepted method is important for detecting fraud and adulteration in honey.
Cane sugar or high fructose corn syrup can be added to honey to reduce its cost. Additional sugar, if any, can be detected with C4 Sugar Analysis within the scope of the AOAC 998.12 standard. Isotope ratio mass spectroscopy is used.
The 13C/12C ratio of honey produced naturally from nectar is different from the 13C/12C ratio that comes from cane sugar or added sugar. According to international evaluations, the difference in the 13C/12C ratio between whole honey and protein (Delta 13C) in unadulterated honey should be less than or equal to 7%.
Consult Nanolab Food Analysis Laboratory for Delta C13 Values and C4 Sugar Analysis in Honey.