Detection and Assay of Riboflavin (Vitamin B2)

BS EN 14152 standard covers the test method for the determination of vitamin B2 (riboflavin) in foodstuffsby high performance liquid chromatography (HPLC). Riboflavin measurement is performed in samples to determine vitamin B2 (riboflavin).

Riboflavin in the sample is determined by acid hydrolysis followed by dephosphorylation using an enzymatic process via high-performance liquid chromatographic (HPLC) separation with fluorometric detection.

Vitamin B2 (riboflavin) analysis, grain, flour, It can be made in pastries and confectionery, fresh milk and dried dairy products, and various vegetables.

BS EN 14152: Foodstuffs — Determination of vitamin B2 by HPLC