Determination of Vitamin B2: Riboflavin Analysis and Importance in Foods

Properties of Vitamin B2

What is Vitamin B2 (Riboflavin)?

Vitamin B2, also known as riboflavin, is a structural compound of the coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are present in the body in active form. Riboflavin is a water-soluble vitamin and is essential for energy production in the body. It is also of great importance for the healthy functioning of cells and the functioning of the antioxidant defense system.

Health Benefits of Riboflavin

Energy production: Involved in the metabolism of carbohydrates, fats and proteins.
Antioxidant effect: Helps protect cells from free radicals.
Growth and development: Essential for skin, hair and eye health.
Nervous system health: Important for brain and nervous system functions.

Determination of Riboflavin in Foods: EN 14152 Standard

Riboflavin is often found in low concentrations in foods, so it is important to use accurate measurement methods. The analysis of riboflavin in food products is a critical step to ensure the accuracy of the nutritional values of food products, to monitor production processes and for product labeling.

EN 14152 is the European standard and specifies the analytical methods used for the determination of riboflavin in foods. This standard provides specifications for accurately measuring the amount of riboflavin in foods. According to EN 14152, riboflavin is usually determined by fluorescence spectrometry or high performance liquid chromatography (HPLC).

Application Areas of Riboflavin Determination

Food Production and Control: Riboflavin determination is performed to monitor riboflavin content in food products, to ensure correct labeling and quality.
Nutrition and Health Studies: Riboflavin levels are particularly important for assessing the nutritional status of vulnerable groups such as children and the elderly.
Compliance with Standards: The EN 14152 standard ensures that food manufacturers and laboratories comply with international quality requirements.

Riboflavin Determination Methods According to EN 14152 Standard

1. Fluorescence Spectrometry Fluorescence spectrometry is a method utilizing the light absorption and emission properties of riboflavin. Riboflavin absorbs light at certain wavelengths and emits light at a different wavelength. This property is used to detect riboflavin.

2. High Performance Liquid Chromatography (HPLC) HPLC is a sensitive method widely used to separate and determine riboflavin in foods. This method allows riboflavin to be separated from other compounds and measured in a pure form. The determination of riboflavin using HPLC is usually complemented by a fluorescence detector.

Nano-lab Laboratories Group continues to provide services within the scope of Vitamin B2 Determination. We also provide services in Vitamin Analysis.

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