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Determination of Aspartame, Aceslulfam – K, Saccharin

Determination of Aspartame, Aceslulfam – K, Saccharin

Determination of Aspartame, Aceslulfam – K, Saccharin


Sweeteners used in foods are generally classified in 2 ways:


  • Sweeteners with nutritional value. Sweeteners with nutritional value contain carbohydrates and provide energy.
  • Non-nutritive sweeteners. Non-nutritive sweeteners, on the other hand, do not provide energy and give a sweet taste, although they do not affect the blood glucose value.


In general, low-calorie or non-calorie sweeteners have been approved by authorities such as FDA and EFSA and are considered safe to use. Today, sweeteners are used in many foodstuffs. Some of these sweeteners can be listed as follows:

  • Aspartame
  • Acesulfame-K
  • Saccharin


Aspartame: The daily intake limit (ADI) of aspartame, the methyl ester of aspartic acid and dipeptide phenylalanine, has been determined as 40 mg/kg. Its sweetness is 200 times higher than sucrose.

Acesulfame K: The shelf life of Acesulfame K, whose daily intake limit (ADI) is determined as 9 mg/kg, is almost unlimited at room temperature. Its sweetness is 300 times higher than sucrose.

Saccharin: The daily intake limit (ADI) of saccharin, which is one of the oldest non-caloric sweeteners allowed for use in foods, has been determined as 15 mg/kg. Its sweetness is 300-500 times higher than sucrose.

Sweeteners such as Aspartame, Aceslulfam - K, Saccharin are frequently used in food and beverages. They are also tested within the scope of food analyzes for quality tests, presence and absence analyzes of additives and verification of label information.

“Determination of Aspartame, Aceslülfam – K, Saccharin” is one of the analyzes performed safely in accredited and authorized Food Analysis Laboratories.

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