Determination of Proline in Honey

What is proline?

One of the 20 amino acids that make up proteins is proline. Unlike other amino acids, it lacks a primary amine group (-NH2), as the three carbon atoms in its side chain form a ring and bond back to the nitrogen atom in the peptide bond. It contains nitrogen (nitrogen), which is called secondary amine.

Benefits of high proline raw honey:
• Thanks to its antimicrobial and antioxidant activities, proline stimulates the immune system. It creates an anti-inflammatory effect.
• It accelerates the healing of wounds and burns.

Proline is an important parameter in honey analysis. The amount of proline can be checked to detect real honey. Honey is a source of protein. Proline is also detected to determine the purity of honey. Proline is an amino acid that is only found in honey and cannot be added externally.

The purpose of Proline Determination is:
• To determine the authenticity of honey
• To determine its quality
• To determine antioxidant activities

In honey within the scope of Turkish Food Codex While the required proline value was 180 mg/kg, it was increased to 300 mg/kg in 2013.

The proline value in quality honey should be 450-500 mg/kg.