Evaluation of Mycobactericidal and Tuberculocidal Activity: Antimicrobial Activity Tests

Evaluation of Mycobactericidal and Tuberculocidal Activity in Biocidal Products

What is Mycobactericidal and Tuberculocidal Activity?

Mycobactericidal Activity: Refers to the activity of chemical or biological agents that have a lethal effect against mycobacteria. Mycobactericidal products have been developed to kill mycobacteria species such as Mycobacterium tuberculosis, Mycobacterium avium.

Tuberculocidal Activity: It means lethal effect againstMycobacterium tuberculosis species. It is especially critical in tuberculosis treatment and infection control.

These activities are tested according to international standards to verify the efficacy of antimicrobial products and to ensure their safe use in healthcare facilities.

What are the Purposes of the Test?

1. Efficacy of Disinfectants: To determine whether disinfectants used in healthcare facilities are effective against mycobacteria, particularly to prevent the spread of tuberculosis.
2. Evaluation of Therapeutic Products: To test the lethal efficacy of drugs used against mycobacterial infections.
3. Risk Analysis: Identifying products with insufficient efficacy against mycobacteria and developing appropriate alternatives.

Standards Used in Testing Around the World

• EN 14348: The most widely used European standard for mycobactericidal and tuberculocidal efficacy testing.
• AOAC 965.12: Another widely used standard in the USA, especially for tuberculocidal efficacy testing.
• CLSI M24-A: A protocol for evaluating the effect of antimicrobial agents on mycobacteria.
• ISO 17025: Standard for quality management system for test methods of laboratories.

Evaluation of Disinfectants

Mycobactericidal disinfectants are often used in the healthcare sector and their effectiveness is tested according to the following factors

• Concentration: It is important to use disinfectants at the correct concentration.
• Duration of Action: Mycobactericidal activity usually depends on the contact time of the agent with mycobacteria.
• Ambient Conditions: Factors such as pH, temperature and the presence of organic matter can affect the effectiveness of disinfectants.

Evaluation of Results

Successful Effectiveness Criteria:

• Meeting logarithmic reduction criteria.
• Complete inhibition or killing of mycobacteria growth.

Confirmation of Availability:

• Test results are reported to certify that antimicrobial products are mycobactericidal or tuberculocidal.
• Products with unsatisfactory results cannot be used in healthcare facilities.

Nanolab Laboratories Group continues to provide services within the scope of Evaluation of Mycobactericidal and Tuberculocidal Activity. We also provide services in Irritation Analysis.

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