Nuclear Magnetic Resonance Spectroscopy of Proteins

Nuclear Magnetic Resonance (NMR) Spectroscopy is used to determine the atomic resolution structures of proteins. A separate signal can be received for each atom in the protein.

Protein NMR structural determination involves four steps:
• Isotope labeled protein sample preparation,
• NMR data collection and analysis, especially the chemical shifts of ¹H, ¹⁵N and ¹³C atoms in the protein molecule assignment,
• Structural calculation and refinement using distance and/or orientation constraints obtained from specific NMR experiments, and
• Structural quality assessment, each comprehensively introduced and reviewed.

With the Nuclear Magnetic Resonance (NMR) method;
• Developing small molecules that can possibly be used to investigate the normal biology of the interaction,
• To identify how two proteins interact with each other
• To provide possible clues for pharmaceutical use (drug development)